Pro-opiomelanocortin gene expression and peptide secretion in human small-cell lung cancer cell lines.
J Mol Endocrinol
; 3(1): 65-70, 1989 Jul.
Article
en En
| MEDLINE
| ID: mdl-2472813
ABSTRACT
Expression of the RNA coding for the ACTH-beta-lipotrophin precursor, pro-opiomelanocortin (POMC), has been demonstrated in five human small-cell lung cancer (SCLC) cell lines. Using Northern and slot-blot hybridization analysis of RNA and a bovine POMC cDNA as probe, the processed POMC RNA from SCLC cells was found to be approximately 1350 nucleotides in length, which is larger than that found in the normal human pituitary. Expression of the POMC gene was confirmed by measurement of ACTH precursors secreted by the cells, using a novel two-site immunoradiometric assay based on monoclonal antibodies, which directly quantifies both POMC and pro-ACTH but does not recognize ACTH. Levels of POMC in medium accumulated throughout the growth of the cells, in contrast to POMC RNA which showed a relatively constant level of expression. We conclude that human SCLC cell lines are valuable models for studying the aberrant expression and regulation of the human POMC gene.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Precursores de Proteínas
/
Proopiomelanocortina
/
Regulación de la Expresión Génica
/
Carcinoma de Células Pequeñas
/
Hormona Adrenocorticotrópica
/
Neoplasias Pulmonares
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Mol Endocrinol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
ENDOCRINOLOGIA
Año:
1989
Tipo del documento:
Article