[Determining the tumor-cell density required for macroscopic observation of 5-ALA-induced fluorescence of protoporphyrin IX in cultured glioma cells and clinical cases].
No Shinkei Geka
; 42(6): 531-6, 2014 Jun.
Article
en Ja
| MEDLINE
| ID: mdl-24920740
ABSTRACT
We investigated the association between the cell density and intensity of 5-aminolevulinic acid-induced fluorescence of protoporphyrin IX in 3-dimensionally cultured C6 glioma cells. The ratio between 636-nm red fluorescence excited by a 405-nm laser and 513-nm green autofluorescence of the tissue was measured as the fluorescence intensity. A ratio exceeding 0.68 was macroscopically judged as fluorescence-positive by observers, and the cell density at this fluorescence intensity was 1×107< cells/mL. In clinical surgical fields, the fluorescence ratio was about 0.65 in cases judged as fluorescence-positive, similarly to that in cultured cells. Neurosurgeons and pathologists should recognize that tumor cells are present in fluorescence-negative regions. It is necessary to develop a device which measures fluorescence more simply than macroscopic observation for cutting-edge brain surgery.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Protoporfirinas
/
Neoplasias Encefálicas
/
Fármacos Fotosensibilizantes
/
Glioma
/
Ácido Aminolevulínico
Límite:
Adult
/
Aged
/
Animals
/
Female
/
Humans
/
Male
Idioma:
Ja
Revista:
No Shinkei Geka
Año:
2014
Tipo del documento:
Article