Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina.
Rev Argent Microbiol
; 46(2): 119-21, 2014.
Article
en En
| MEDLINE
| ID: mdl-25011595
ABSTRACT
Different species of Mycoplasma can affect bovine cattle, causing several diseases. PCR sequencing and further analysis of the 16S-23S rRNA ITS region have shown a significant interspecies variability among Mollicutes. Sixteen suspected isolates of Mycoplasma spp. obtained from milk samples from dairy herds were amplified (16S-23S rRNA ITS region). Fourteen out of those 16 suspected Mycoplasma spp. isolates were PCR-positive. To confirm the identity of Mycoplasma bovis, these 14 isolates were tested by another species-specific PCR. Seven of the isolates rendered a positive result. The products of 16S-23S rRNA ITS PCR from one isolate that was identified as M. bovis and from two other isolates, identified as non- M. bovis were randomly selected, sequenced and analyzed. The three sequences (A, B and C) showed 100% similarity with M. bovis, Mycoplasma canadense and Mycoplasma californicum respectively.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bovinos
/
Leche
/
Mycoplasma
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
País/Región como asunto:
America do sul
/
Argentina
Idioma:
En
Revista:
Rev Argent Microbiol
Año:
2014
Tipo del documento:
Article