Improved stability and half-life of fluorinated phosphotriesterase using Rosetta.
Chembiochem
; 15(12): 1761-4, 2014 Aug 18.
Article
en En
| MEDLINE
| ID: mdl-25066940
ABSTRACT
Recently we demonstrated that incorporating p-fluorophenylalanine (pFF) into phosphotriesterase dramatically improved folding, thereby leading to enhanced stability and function at elevated temperatures. To further improve the stability of the fluorinated enzyme, Rosetta was used to identify multiple potential stabilizing mutations. One such variant, pFF-F104A, exhibited enhanced activity at elevated temperature and maintained activity over many days in solution at room temperature.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
P-Fluorofenilalanina
/
Hidrolasas de Triéster Fosfórico
Idioma:
En
Revista:
Chembiochem
Asunto de la revista:
BIOQUIMICA
Año:
2014
Tipo del documento:
Article