Isoelectric focusing in a silica nanofluidic channel: effects of electromigration and electroosmosis.
Anal Chem
; 86(17): 8711-8, 2014 Sep 02.
Article
en En
| MEDLINE
| ID: mdl-25098739
ABSTRACT
Isoelectric focusing of proteins in a silica nanofluidic channel filled with citric acid and disodium phosphate buffers is investigated via numerical simulation. Ions in the channel migrate in response to (i) the electric field acting on their charge and (ii) the bulk electroosmotic flow (which is directed toward the cathode). Proteins are focused near the low pH (anode) end when the electromigration effect is more significant and closer to the high pH (cathode) end when the electroosmotic effect dominates. We simulate the focusing behavior of Dylight labeled streptavidin (Dyl-Strep) proteins in the channel, using a relationship between the protein's charge and pH measured in a previous experiment. Protein focusing results compare well to previous experimental measurements. The effect of some key parameters, such as applied voltage, isoelectric point (pI), bulk pH, and bulk conductivity, on the protein trapping behavior in a nanofluidic channel is examined.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas
/
Dióxido de Silicio
/
Nanotecnología
Idioma:
En
Revista:
Anal Chem
Año:
2014
Tipo del documento:
Article
País de afiliación:
Australia