Evaluation of UV-HPLC and mass spectrometry methods for specific activity determination.
J Labelled Comp Radiopharm
; 57(11): 645-51, 2014 Sep.
Article
en En
| MEDLINE
| ID: mdl-25223538
ABSTRACT
The specific activity (SA) values determined using two different methods were compared for a set of tritium-labeled and carbon-14-labeled compounds. The methods employed were as follows (a) liquid chromatography/mass spectrometry (LC/MS) isotopic peak intensity distribution, and (b) determination of the tracer mass concentration using ultraviolet-high-performance liquid chromatography analysis coupled with the radioactive solution concentration measured by liquid scintillation counting. In general, at lower SA, the accuracy and or precision of the LC/MS-determined SA value decreased significantly. Because of this decrease in accuracy, a rough guideline of ~10% of the theoretical maximum SA is recommended as the lower cutoff for MS-based SA measurements. If the tracer contains heteroatoms that possess significant percentages of heavy isotopes at natural abundance (e.g. Cl and Br), then the MS-based SA cutoff recommendation is approximately 25-30% of the fully labeled compound in the tracer mixture. Additionally, IsoPat(2) was found to be the preferred calculation method for LC/MS-based SA determination because SA values via this program were more consistent with those obtained by ultraviolet concentration calibration with solution count.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cromatografía Líquida de Alta Presión
/
Radiofármacos
/
Cromatografía de Gases y Espectrometría de Masas
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
J Labelled Comp Radiopharm
Año:
2014
Tipo del documento:
Article
País de afiliación:
Suecia