Protein hairy enhancer of split-1 expression during differentiation of muscle-derived stem cells into neuron-like cells.
Neural Regen Res
; 7(28): 2182-7, 2012 Oct 05.
Article
en En
| MEDLINE
| ID: mdl-25538738
ABSTRACT
Muscle-derived stem cells were isolated from the skeletal muscle of Sprague-Dawley neonatal rats aged 3 days old. Cells at passage 5 were incubated in Dulbecco's modified Eagle's medium supplemented with 10% (v/v) fetal bovine serum, 20 µg/L nerve growth factor, 20 µg/L basic fibroblast growth factor and 1% (v/v) penicillin for 6 days. Cells presented with long processes, similar to nerve cells. Connections were formed between cell processes. Immunocytochemical staining with neuron specific enolase verified that cells differentiated into neuron-like cells. Immunofluorescence cytochemistry and western blot results revealed that the expression of protein hairy enhancer of split-1 was significantly reduced. These results indicate that low expression of protein hairy enhancer of split-1 participates in the differentiation of muscle-derived stem cells into neuron-like cells.
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1
Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
Neural Regen Res
Año:
2012
Tipo del documento:
Article
País de afiliación:
China