A reproducible method for the expansion of mouse CD8+ T lymphocytes.
J Immunol Methods
; 417: 134-138, 2015 Feb.
Article
en En
| MEDLINE
| ID: mdl-25602136
ABSTRACT
Murine adoptive CD8+ T-cell immunotherapy studies require the generation of large numbers of high viability CD8+ cells. Here we report a tissue culture protocol for the reliable expansion of CD8+ T-cells derived from murine spleen to give a 20-fold expansion after 4 days in culture. The cells were transfected with an mRNA GFP construct and transferred into NOD mice. GFP positive cells could be detected 7 days after transfer thus confirming that the cells survive and are functional for up to 1 week.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Inmunoterapia Adoptiva
/
Linfocitos T CD8-positivos
/
Técnicas de Cultivo de Célula
Límite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Año:
2015
Tipo del documento:
Article
País de afiliación:
Reino Unido