Indole-based cyanine as a nuclear RNA-selective two-photon fluorescent probe for live cell imaging.
ACS Chem Biol
; 10(5): 1171-5, 2015 May 15.
Article
en En
| MEDLINE
| ID: mdl-25689264
ABSTRACT
We have demonstrated that the subcellular targeting properties of the indole-based cyanines can be tuned by the functional substituent attached onto the indole moiety in which the first example of a highly RNA-selective and two-photon active fluorescent light-up probe for high contrast and brightness TPEF images of rRNA in the nucleolus of live cells has been developed. It is important to find that this cyanine binds much stronger toward RNA than DNA in a buffer solution as well as selectively stains and targets to rRNA in the nucleolus. Remarkably, the TPEF brightness (Φσmax) is dramatically increased with 11-fold enhancement in the presence of rRNA, leading to the record high Φσmax of 228 GM for RNA. This probe not only shows good biocompatibility and superior photostability but also offers general applicability to various live cell lines including HeLa, HepG2, MCF-7, and KB cells and excellent counterstaining compatibility with commercially available DNA or protein trackers.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ARN Nuclear
/
Colorantes Fluorescentes
/
Indoles
Límite:
Humans
Idioma:
En
Revista:
ACS Chem Biol
Año:
2015
Tipo del documento:
Article
País de afiliación:
China