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Malaria. A forward genetic screen identifies erythrocyte CD55 as essential for Plasmodium falciparum invasion.
Egan, Elizabeth S; Jiang, Rays H Y; Moechtar, Mischka A; Barteneva, Natasha S; Weekes, Michael P; Nobre, Luis V; Gygi, Steven P; Paulo, Joao A; Frantzreb, Charles; Tani, Yoshihiko; Takahashi, Junko; Watanabe, Seishi; Goldberg, Jonathan; Paul, Aditya S; Brugnara, Carlo; Root, David E; Wiegand, Roger C; Doench, John G; Duraisingh, Manoj T.
Afiliación
  • Egan ES; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA, USA. Division of Infectious Diseases, Boston Children's Hospital, Boston, MA, USA.
  • Jiang RH; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA, USA. Department of Global Health and Center for Drug Discovery and Innovation, University of South Florida, Tampa, FL, USA.
  • Moechtar MA; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA, USA.
  • Barteneva NS; Department of Pediatrics, Harvard Medical School and Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA.
  • Weekes MP; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.
  • Nobre LV; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.
  • Gygi SP; Department of Cell Biology, Harvard Medical School, Boston, MA, USA.
  • Paulo JA; Department of Cell Biology, Harvard Medical School, Boston, MA, USA.
  • Frantzreb C; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA, USA.
  • Tani Y; Japanese Red Cross Kinki Block Blood Center, Osaka, Japan.
  • Takahashi J; Japanese Red Cross Kinki Block Blood Center, Osaka, Japan.
  • Watanabe S; Japanese Red Cross Kyushu Block Blood Center, Fukuoka, Japan.
  • Goldberg J; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA, USA.
  • Paul AS; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA, USA.
  • Brugnara C; Department of Laboratory Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
  • Root DE; The Broad Institute of Harvard and Massachussetts Insititute of Technology, Cambridge, MA, USAA.
  • Wiegand RC; The Broad Institute of Harvard and Massachussetts Insititute of Technology, Cambridge, MA, USAA.
  • Doench JG; The Broad Institute of Harvard and Massachussetts Insititute of Technology, Cambridge, MA, USAA.
  • Duraisingh MT; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, MA, USA. The Broad Institute of Harvard and Massachussetts Insititute of Technology, Cambridge, MA, USAA. mduraisi@hsph.harvard.edu.
Science ; 348(6235): 711-4, 2015 May 08.
Article en En | MEDLINE | ID: mdl-25954012
ABSTRACT
Efforts to identify host determinants for malaria have been hindered by the absence of a nucleus in erythrocytes, which precludes genetic manipulation in the cell in which the parasite replicates. We used cultured red blood cells derived from hematopoietic stem cells to carry out a forward genetic screen for Plasmodium falciparum host determinants. We found that CD55 is an essential host factor for P. falciparum invasion. CD55-null erythrocytes were refractory to invasion by all isolates of P. falciparum because parasites failed to attach properly to the erythrocyte surface. Thus, CD55 is an attractive target for the development of malaria therapeutics. Hematopoietic stem cell-based forward genetic screens may be valuable for the identification of additional host determinants of malaria pathogenesis.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Plasmodium falciparum / Malaria Falciparum / Antígenos CD55 / Eritrocitos / Interacciones Huésped-Parásitos Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Science Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
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PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Plasmodium falciparum / Malaria Falciparum / Antígenos CD55 / Eritrocitos / Interacciones Huésped-Parásitos Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Science Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos