Whole-Slide Imaging of Pap Cellblock Preparations Is a Potentially Valid Screening Method.
Acta Cytol
; 59(2): 187-200, 2015.
Article
en En
| MEDLINE
| ID: mdl-25967603
ABSTRACT
OBJECTIVE:
To date, the impact of digital imaging on routine cytology remains far from perfect. Cellblock (CB) preparations from Pap samples have been shown to be diagnostically valuable. We evaluated the validity of utilizing whole-slide imaging (WSI) prepared from Pap CBs as a screening tool. STUDYDESIGN:
A total of 1,110 CB slides prepared from residual Pap samples were analyzed - 563 normal, 282 atypical squamous cells of undetermined significance (ASCUS), 12 atypical squamous cells-cannot exclude high-grade squamous intraepithelial lesion, 188 low-grade squamous intraepithelial lesions (LSIL), 36 high-grade squamous intraepithelial lesions (HSIL), 25 atypical glandular cells of undetermined significance, 1 adenocarcinoma in situ, 2 invasive adenocarcinomas, and 1 squamous cell carcinoma. Virtual slides were obtained using the Aperio system. Test performance characteristics of liquid-based samples and WSI from CB samples were compared.RESULTS:
Average sensitivity and specificity of the five WSI reviewers was 58.3 and 85.1% for ASCUS, respectively, 54.1 and 93.9% for LSIL, and 51.8 and 98.8% for HSIL. Overall WSI sensitivity and specificity for detecting lesions was 82.1 and 86.2%, respectively. Agreement (kappa values) between WSI reviewers was 0.56 for ASCUS, 0.69 for LSIL, 0.67 for HSIL, and 0.74 for negative samples.CONCLUSIONS:
WSI of CB preparations is a feasible method to achieve high-quality specimen preparations. It is as sensitive as liquid-based methods and appears to be highly specific for the detection of LSIL and HSIL.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Frotis Vaginal
/
Interpretación de Imagen Asistida por Computador
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Carcinoma de Células Escamosas
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Adenocarcinoma
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Displasia del Cuello del Útero
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Neoplasias del Cuello Uterino
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Cuello del Útero
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Prueba de Papanicolaou
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Células Escamosas Atípicas del Cuello del Útero
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Lesiones Intraepiteliales Escamosas de Cuello Uterino
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
/
Screening_studies
Límite:
Adolescent
/
Adult
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Aged
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Aged80
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Female
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Humans
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Middle aged
Idioma:
En
Revista:
Acta Cytol
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos