The genetic toolbox for Acidovorax temperans.
J Microbiol Methods
; 115: 129-38, 2015 Aug.
Article
en En
| MEDLINE
| ID: mdl-26079987
ABSTRACT
Environmental isolates belonging to the genus Acidovorax play a crucial role in degrading a wide range of pollutants. Studies on Acidovorax are currently limited for many species due to the lack of genetic tools. Here, we described the use of the replicon from a small, cryptic plasmid indigenous to Acidovorx temperans strain CB2, to generate stably maintained shuttle vectors. In addition, we have developed a scarless gene knockout technique, as well as establishing green fluorescent protein (GFP) reporter and complementation systems. Taken collectively, these tools will improve genetic manipulations in the genus Acidovorax.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Genéticas
/
Comamonadaceae
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
J Microbiol Methods
Año:
2015
Tipo del documento:
Article