The effect of silencing arginine kinase by RNAi on the larval development of Helicoverpa armigera.
Bull Entomol Res
; 105(5): 555-65, 2015 Oct.
Article
en En
| MEDLINE
| ID: mdl-26138927
ABSTRACT
Arginine kinase (AK) is an important regulation factor of energy metabolism in invertebrate. An arginine kinase gene, named HaAK, was identified to be differentially expressed between Cry1Ac-susceptible (96S) and Cry1Ac-resistant (Bt-R) Helicoverpa armigera larvae using cDNA-amplification fragment length polymorphism analysis. The full-length open reading frame sequence of HaAK gene with 1068 bp was isolated from H. armigera. Quantitative reverse transcription polymerase chain reaction assay revealed that HaAK gene is specifically expressed in multiple tissues and at larval developmental stages. The peak expression level of HaAK was detected in the midgut of the fifth-instar larvae. Moreover, the expression of HaAK was obviously down-regulated in Bt-R larvae. We further constructed a dsRNA vector directly targeting HaAK and employed RNAi technology to control the larvae. The feeding bioassays showed that minute quantities of dsRNA could greatly increase the larval mortality and delay the larval pupation. Silencing of HaAK significantly retarded the larval development, indicating that HaAK is a potential target for RNA interference-based pest management.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Arginina Quinasa
/
Regulación Enzimológica de la Expresión Génica
/
Regulación del Desarrollo de la Expresión Génica
/
Interferencia de ARN
/
Mariposas Nocturnas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Bull Entomol Res
Año:
2015
Tipo del documento:
Article
País de afiliación:
China