Highmobility group box 1 has a prognostic role and contributes to epithelial mesenchymal transition in human hepatocellular carcinoma.
Mol Med Rep
; 12(4): 5997-6004, 2015 Oct.
Article
en En
| MEDLINE
| ID: mdl-26252226
ABSTRACT
Highmobility group box 1 (HMGB1), a member of the highmobility group protein family, was originally characterized as a nonhistone, nuclear DNAbinding protein. While the roles of HMGB1 in inflammation and cell differentiation have been previously reported, its role in tumor cell migration and invasion, particularly in hepatocellular carcinoma (HCC), has remained elusive. The present study reported that the expression of HMGB1 in HCC tissues was significantly higher than that in matched tumoradjacent tissues (P<0.05). HMGB1 was expressed at significantly elevated levels in tumors of patients with large tumor size, high histological grade and advanced tumornodemetastasis stage (P<0.05). The positive expression of HMGB1 correlated with a poor threeyear overall and diseasefree survival of HCC patients (P<0.05). In addition, HMGB1 was an independent factor for predicting the threeyear overall and diseasefree survival of HCC patients (P<0.05). An in vitro experiment revealed that knockdown of HMGB1 inhibited cell migration and invasion in the HCC cell lines Huh7 and MHCC97H (P<0.05). Furthermore, western blot analysis showed that HMGB1 knockdown markedly inhibited epithelial mesenchymal transition in Huh7 and MHCC97H cells. These results suggested that HMGB1 may be utilized as an independent prognostic marker in HCC and may promote tumor progression by promoting cell migration and invasion.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Regulación Neoplásica de la Expresión Génica
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Carcinoma Hepatocelular
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Proteína HMGB1
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Transición Epitelial-Mesenquimal
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Neoplasias Hepáticas
Tipo de estudio:
Prognostic_studies
Límite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Mol Med Rep
Año:
2015
Tipo del documento:
Article