Catalpol downregulates vascular endothelialcadherin expression and induces vascular hyperpermeability.
Mol Med Rep
; 13(1): 373-8, 2016 Jan.
Article
en En
| MEDLINE
| ID: mdl-26549479
ABSTRACT
Catalpol, an iridiod glucoside isolated from Rehmannia glutinosa, has been reported to possess antiinflammatory properties. However, the molecular mechanisms underlying this effect have not been fully elucidated. This study aimed to investigate the effects of catalpol on vascular permeability. Using Transwell permeability assays and measurements of transendothelial electrical resistance (TEER), it was demonstrated that 1 mM catalpol induces a significant increase in the permeability of the monolayers of human umbilical vein endothelial cells (HUVECs). Western blotting and immunofluorescence demonstrated that catalpol inhibits the expression of vascular endothelial (VE)cadherin, the key component of adherens junctions, but not occludin, the major constituent of tight junctions. In addition, catalpol inhibits the ETS transcription factor ERG, a positive regulator of VEcadherin. Knockdown of ERG expression compromised the catalpolinduced reduction of TEER in HUVECs. The present study revealed a novel effect of catalpol on vascular permeability and gave insight into the multifaceted roles of catalpol in inflammation.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Permeabilidad Capilar
/
Endotelio Vascular
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Antígenos CD
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Cadherinas
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Regulación hacia Abajo
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Glucósidos Iridoides
Límite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Año:
2016
Tipo del documento:
Article