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Functional and structural characterization of a heparanase.
Bohlmann, Lisa; Tredwell, Gregory D; Yu, Xing; Chang, Chih-Wei; Haselhorst, Thomas; Winger, Moritz; Dyason, Jeffrey C; Thomson, Robin J; Tiralongo, Joe; Beacham, Ifor R; Blanchard, Helen; von Itzstein, Mark.
Afiliación
  • Bohlmann L; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
  • Tredwell GD; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
  • Yu X; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
  • Chang CW; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
  • Haselhorst T; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
  • Winger M; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
  • Dyason JC; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
  • Thomson RJ; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
  • Tiralongo J; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
  • Beacham IR; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
  • Blanchard H; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
  • von Itzstein M; Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
Nat Chem Biol ; 11(12): 955-7, 2015 Dec.
Article en En | MEDLINE | ID: mdl-26565989
ABSTRACT
We report the structural and functional characterization of a novel heparanase (BpHep) from the invasive pathogenic bacterium Burkholderia pseudomallei (Bp), showing ∼24% sequence identity with human heparanase (hHep). Site-directed mutagenesis studies confirmed the active site resi-dues essential for activity, and we found that BpHep has specificity for heparan sulfate. Finally, we describe the first heparanase X-ray crystal structure, which provides new insight into both substrate recognition and inhibitor design.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Burkholderia pseudomallei / Glucuronidasa Límite: Humans Idioma: En Revista: Nat Chem Biol Asunto de la revista: BIOLOGIA / QUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Australia
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Burkholderia pseudomallei / Glucuronidasa Límite: Humans Idioma: En Revista: Nat Chem Biol Asunto de la revista: BIOLOGIA / QUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Australia