Small Buccal Fat Pad Cells Have High Osteogenic Differentiation Potential.
Tissue Eng Part C Methods
; 22(3): 250-9, 2016 Mar.
Article
en En
| MEDLINE
| ID: mdl-26651216
ABSTRACT
Dedifferentiated fat (DFAT) cells derived from mature adipocytes have mesenchymal stem cells' (MSCs) characteristics. Generally, mature adipocytes are 60-110 µm in diameter; however, association between adipocyte size and dedifferentiation efficiency is still unknown. This study, therefore, investigated the dedifferentiation efficiency of adipocytes based on cell diameter. Buccal fat pad was harvested from five human donors and dissociated by collagenase digestion. After exclusion of unwanted stromal cells by centrifugation, floating adipocytes were collected and their size distribution was analyzed. The floating adipocytes were then separated into two groups depending on cell size using 40- and 100-µm nylon mesh filters cell diameters less than 40 µm (small adipocytes S-adipocytes) and cell diameters of 40-100 µm (large adipocytes L-adipocytes). Finally, we evaluated the efficiency of adipocyte dedifferentiation and then characterized the resultant DFAT cells. The S-adipocytes showed a higher capacity to dedifferentiate into DFAT cells (S-DFAT cells) compared to the L-adipocytes (L-DFAT cells). The S-DFAT cells also showed a relatively higher proportion of CD146-positive cells than L-DFAT cells, and exhibited more osteogenic differentiation ability based on the alkaline phosphatase activity and amount of calcium deposition. These results suggested that the S- and L-DFAT cells had distinct characteristics, and that the higher dedifferentiation potential of S-adipocytes compared to L-adipocytes gives the former group an advantage in yielding DFAT cells.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Osteogénesis
/
Diferenciación Celular
/
Tejido Adiposo
/
Boca
Límite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Tissue Eng Part C Methods
Asunto de la revista:
BIOTECNOLOGIA
/
HISTOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Japón