A study of microRNAs from dried blood spots in newborns after perinatal asphyxia: a simple and feasible biosampling method.
Pediatr Res
; 79(5): 799-805, 2016 05.
Article
en En
| MEDLINE
| ID: mdl-26720606
ABSTRACT
BACKGROUND:
The potential of microRNAs (miRNAs) as bedside biomarkers in selecting newborns with hypoxic-ischemic encephalopathy (HIE) for neuroprotection has yet to be explored. Commonly, blood-based biomarker tests use plasma or serum which don't allow evaluation of both intracellular and extracellular changes.METHODS:
We describe a technique to extract and compare expression of miRNAs from a single small 6-mm-diameter dried blood spot (DBS) stored at room temperature with those from EDTA-blood, plasma, and urine. Three miRNAs (RNU6B, let7b, and miR-21) were quantified via extraction and quantitative RT-PCR performed from a DBS and compared with levels from EDTA-blood, plasma, and urine. Secondarily, candidate miRNAs let7b, miR-21, miR-29b, miR-124, and miR-155 in DBS were evaluated as potential biomarkers for HIE.RESULTS:
Candidate miRNAs were extractable in all biosamples from newborns, with the highest expression in DBS. There was a good correlation between miRNAs' levels in DBS and EDTA-blood at -80 °C. No significant difference was observed in the miRNA levels between the favorable and unfavorable outcome groups for babies with HIE.CONCLUSION:
DBS may be useful for studying the potential of miRNAs as biomarkers for brain injury.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Asfixia Neonatal
/
Tamizaje Neonatal
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MicroARNs
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Pruebas con Sangre Seca
Límite:
Female
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Humans
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Male
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Newborn
Idioma:
En
Revista:
Pediatr Res
Año:
2016
Tipo del documento:
Article
País de afiliación:
Reino Unido