Obesity decreases B cell responses in young and elderly individuals.
Obesity (Silver Spring)
; 24(3): 615-25, 2016 Mar.
Article
en En
| MEDLINE
| ID: mdl-26857091
ABSTRACT
OBJECTIVE:
To evaluate the effects of obesity-associated inflammation on influenza vaccine responses.METHODS:
In young and elderly individuals, both lean and with obesity, antibody responses to influenza vaccination were measured.RESULTS:
A decrease in in vivo vaccine responses, circulating switched memory, and transitional B cells and an increase in pro-inflammatory late/exhausted memory B cells were found. In vitro B cell function was measured by activation-induced cytidine deaminase and E47, markers of optimal antibody responses. Moreover, IL-6 production was increased, whereas IL-10 production was decreased in cultures of B cells from individuals with obesity. Markers of immune activation (TNF-α, TLR4, micro-RNAs) in unstimulated B cells were also found increased and were negatively correlated with B cell function. In order to reveal potential mechanisms, we stimulated B cells from lean individuals in vitro with leptin, the adipokine increased in obesity. Leptin increased phospho-STAT3, crucial for TNF-α production, and decreased phospho-AMPK, the energy sensing enzyme upstream of phospho-p38 MAPK and E47. Leptin-induced phospho-STAT3 and phospho-AMPK levels were similar to those in B cells from individuals with obesity.CONCLUSIONS:
These results demonstrate that leptin can be responsible for decreased B cell function in obesity.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Vacunas contra la Influenza
/
Linfocitos B
/
Activación de Linfocitos
/
Gripe Humana
/
Obesidad
Límite:
Adult
/
Aged
/
Humans
/
Male
Idioma:
En
Revista:
Obesity (Silver Spring)
Asunto de la revista:
CIENCIAS DA NUTRICAO
/
FISIOLOGIA
/
METABOLISMO
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos