Gene characterization and transcription analysis of two new ammonium transporters in pear rootstock (Pyrus betulaefolia).
J Plant Res
; 129(4): 737-748, 2016 Jul.
Article
en En
| MEDLINE
| ID: mdl-26943161
ABSTRACT
Ammonium is the primarily nitrogen source for plant growth, but the molecular basis of ammonium acquisition in fruit species remains poorly understood. In this study, we report on the characterization of two new ammonium transporters (AMT) in the perennial tree Pyrus betulaefolia. In silico analyses and yeast complementation assays revealed that both PbAMT1;3 and PbAMT1;5 can be classified in the AMT1 sub-family. The specific expression of PbAMT1;3 in roots and of PbAMT1;5 in leaves indicates that they have diverse functions in ammonium uptake or transport in P. betulaefolia. Their expression was strongly influenced by ammonium availability. In addition, the transcript level of PbAMT1;5 was significantly affected by the diurnal cycle and senescence hormones. They conferred the ability to uptake nitrogen to the yeast strain 31019b; however, the (15)NH4 (+) uptake kinetics of PbAMT1;3 were different from those of PbAMT1;5. Indeed, PbAMT1;3 had a higher affinity for (15)NH4 (+), and pH changes were associated with this substrates' transport in yeast. The present study provides basic gene features and transcriptional information for the two new members of the AMT1 sub-family in P. betulaefolia and will aid in decoding the precise roles of AMTs in P. betulaefolia physiology.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
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Transcripción Genética
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Genes de Plantas
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Regulación de la Expresión Génica de las Plantas
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Proteínas de Transporte de Catión
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Pyrus
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Compuestos de Amonio
Idioma:
En
Revista:
J Plant Res
Asunto de la revista:
BOTANICA
Año:
2016
Tipo del documento:
Article
País de afiliación:
China