Intervertebral disc regeneration using plateletrich plasmacontaining bone marrowderived mesenchymal stem cells: A preliminary investigation.
Mol Med Rep
; 13(4): 3475-81, 2016 Apr.
Article
en En
| MEDLINE
| ID: mdl-26956080
ABSTRACT
Plateletrich plasma (PRP) is a promising strategy for intervertebral disc degeneration (IDD). However, the short halflife of growth factors released from PRP cannot continuously stimulate the degenerated discs. Thus, the present study hypothesized that the combined use of PRP and bone marrowderived mesenchymal stem cells (BMSCs) may repair the early degenerated discs in the long term for their synergistic reparative effect. In the present study, following the induction of early IDD by annular puncture in rabbits, PRP was prepared and mixed with BMSCs (PRPBMSC group) for injection into the early degenerated discs. As controls, phosphatebuffered saline (PBS; PBS group) and PRP (PRP group) were similarly injected. Rabbits without any intervention served as a control group. At 8 weeks following treatment, histological changes of the injected discs were assessed. Magnetic resonance imaging (MRI) was used to detect the T2weighted signal intensity of the targeted discs at weeks 1, 2 and 8 following treatment. Annular puncture resulted in disc narrowing and decreased T2weighted signal intensity. At weeks 1 and 3, MRI examinations showed regenerative changes in the PRPBMSC group and PRP group, whereas the PBS group exhibited a continuous degenerative process of the discs. At 8 weeks postinjection, the PRPBMSCs induced a statistically significant restoration of discs, as shown by MRI (PRPBMSCs, vs.PRP and PBS; P<0.05), which was also confirmed by histological evaluations. Thus, compared with PRP, the administration of PRPcontaining BMSCs resulted in a superior regenerative effect on the early degenerated discs, which may be a promising therapeutic strategy for the restoration of early degenerated discs.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Trasplante de Células Madre Mesenquimatosas
/
Plasma Rico en Plaquetas
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Degeneración del Disco Intervertebral
/
Células Madre Mesenquimatosas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Año:
2016
Tipo del documento:
Article