Large-scale implementation of sequential protein and peptide immunoaffinity enrichment LC/nanoLC-MS/MS for human ß-nerve growth factor.
Bioanalysis
; 8(8): 753-64, 2016 Apr.
Article
en En
| MEDLINE
| ID: mdl-27005855
ABSTRACT
BACKGROUND:
A previously described immunoaffinity (IA)-LC-MS/MS assay for human ß-nerve growth factor (ß-NGF) was implemented to support large-scale sample testing for multiple clinical trials. Methodology &results:
The procedure was modified to increase throughput by simultaneous preparation of two 96-well plates and LC duty-cycle reduction. Robustness of the LC method and nano-ESI was ensured during large-scale assay execution by closely monitoring and, if needed, replacing system components prior to failure. Following validation, the assay was used to analyze approximately 19,000 samples from multiple clinical studies over several years.CONCLUSION:
Routine implementation of the ß-NGF IA-LC-MS/MS assay supported drug development programs. This optimized assay format now serves as a template for other clinical protein biomarker assays.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cromatografía Líquida de Alta Presión
/
Factor de Crecimiento Nervioso
/
Espectrometría de Masas en Tándem
Límite:
Humans
Idioma:
En
Revista:
Bioanalysis
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos