Hyperspectral multiplex single-particle tracking of different receptor subtypes labeled with quantum dots in live neurons.
J Biomed Opt
; 21(4): 46008, 2016 Apr 30.
Article
en En
| MEDLINE
| ID: mdl-27109870
ABSTRACT
The efficacy of existing therapies and the discovery of innovative treatments for central nervous system (CNS) diseases have been limited by the lack of appropriate methods to investigate complex molecular processes at the synaptic level. To improve our capability to investigate complex mechanisms of synaptic signaling and remodeling, we designed a fluorescence hyperspectral imaging platform to simultaneously track different subtypes of individual neurotransmitter receptors trafficking in and out of synapses. This imaging platform allows simultaneous image acquisition of at least five fluorescent markers in living neurons with a high-spatial resolution. We used quantum dots emitting at different wavelengths and functionalized to specifically bind to single receptors on the membrane of living neurons. The hyperspectral imaging platform enabled the simultaneous optical tracking of five different synaptic proteins, including subtypes of glutamate receptors (mGluR and AMPAR) and postsynaptic signaling proteins. It also permitted the quantification of their mobility after treatments with various pharmacological agents. This technique provides an efficient method to monitor several synaptic proteins at the same time, which could accelerate the screening of effective compounds for treatment of CNS disorders.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Puntos Cuánticos
/
Imagen Molecular
/
Imagen Óptica
/
Colorantes Fluorescentes
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
J Biomed Opt
Asunto de la revista:
ENGENHARIA BIOMEDICA
/
OFTALMOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Canadá