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Ethanol Extract of Cissus quadrangularis Enhances Osteoblast Differentiation and Mineralization of Murine Pre-Osteoblastic MC3T3-E1 Cells.
Tasadduq, Raazia; Gordon, Jonathan; Al-Ghanim, Khalid A; Lian, Jane B; Van Wijnen, Andre J; Stein, Janet L; Stein, Gary S; Shakoori, Abdul Rauf.
Afiliación
  • Tasadduq R; School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.
  • Gordon J; Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vemont.
  • Al-Ghanim KA; Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vemont.
  • Lian JB; Department of Zoology, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
  • Van Wijnen AJ; Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vemont.
  • Stein JL; Department of Orthopedic Surgery and Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota.
  • Stein GS; Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vemont.
  • Shakoori AR; Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vemont.
J Cell Physiol ; 232(3): 540-547, 2017 Mar.
Article en En | MEDLINE | ID: mdl-27264191
Traditional medicinal literature and previous studies have reported the possible role of Cissus quadrangularis (CQ) as an anti-osteoporotic agent. This study examines the effectiveness of CQ in promoting osteoblast differentiation of the murine pre-osteoblast cell line, MC3T3-E1. Ethanolic extract of CQ (CQ-E) was found to affect growth kinetics of MC3T3-E1 cells in a dosage-dependent manner. High concentrations of CQ-E (more than 10 µg/ml) have particularly adverse effects, while lower concentrations of 0.1 and 1 µg/ml were non-toxic and did not affect cell viability. Notably, cell proliferation was significantly increased at the lower concentrations of CQ-E. CQ-E treatment also augmented osteoblast differentiation, as reflected by a substantial increase in expression of the early osteoblast marker ALP activity, and at later stage, by mineralization of extracellular matrix compared to the control group. These findings suggest dose-dependent effect of CQ-E with lower concentrations exhibiting anabolic and osteogenic properties. J. Cell. Physiol. 232: 540-547, 2017. © 2016 Wiley Periodicals, Inc.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Osteoblastos / Calcificación Fisiológica / Extractos Vegetales / Diferenciación Celular / Cissus / Etanol Límite: Animals Idioma: En Revista: J Cell Physiol Año: 2017 Tipo del documento: Article País de afiliación: Pakistán

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Osteoblastos / Calcificación Fisiológica / Extractos Vegetales / Diferenciación Celular / Cissus / Etanol Límite: Animals Idioma: En Revista: J Cell Physiol Año: 2017 Tipo del documento: Article País de afiliación: Pakistán