Quantitative phosphoproteomic analysis of the PI3K-regulated signaling network.
Proteomics
; 16(14): 1992-7, 2016 07.
Article
en En
| MEDLINE
| ID: mdl-27282143
ABSTRACT
The PI3K pathway is commonly activated in cancer. Only a few studies have attempted to explore the spectrum of phosphorylation signaling downstream of the PI3K cascade. Such insight, however, is imperative to understand the mechanisms responsible for oncogenic phenotypes. By applying MS-based phosphoproteomics, we mapped 2509 phosphorylation sites on 1096 proteins, and quantified their responses to activation or inhibition of PIK3CA using isogenic knock-in derivatives and a series of targeted inhibitors. We uncovered phosphorylation changes in a wide variety of proteins involved in cell growth and proliferation, many of which have not been previously associated with PI3K signaling. A significant update of the posttranslational modification database PHOSIDA (http//www.phosida.com) allows efficient use of the data. All MS data have been deposited in the ProteomeXchange with identifier PXD003899 (http//proteomecentral.proteomexchange.org/dataset/PXD003899).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfoproteínas
/
Regulación Neoplásica de la Expresión Génica
/
Transformación Celular Neoplásica
/
Procesamiento Proteico-Postraduccional
/
Fosfatidilinositol 3-Quinasas
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Células Epiteliales
/
Proteínas de Neoplasias
Límite:
Humans
Idioma:
En
Revista:
Proteomics
Asunto de la revista:
BIOQUIMICA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos