Membrane-bound electron transport systems of an anammox bacterium: A complexome analysis.
Biochim Biophys Acta
; 1857(10): 1694-704, 2016 10.
Article
en En
| MEDLINE
| ID: mdl-27461995
ABSTRACT
Electron transport, or oxidative phosphorylation, is one of the hallmarks of life. To this end, prokaryotes evolved a vast variety of protein complexes, only a small part of which have been discovered and studied. These protein complexes allow them to occupy virtually every ecological niche on Earth. Here, we applied the method of proteomics-based complexome profiling to get a better understanding of the electron transport systems of the anaerobic ammonium-oxidizing (anammox) bacteria, the N2-producing key players of the global nitrogen cycle. By this method nearly all respiratory complexes that were previously predicted from genome analysis to be involved in energy and cell carbon fixation were validated. More importantly, new and unexpected ones were discovered. We believe that complexome profiling in concert with (meta)genomics offers great opportunities to expand our knowledge on bacterial respiratory processes at a rapid and massive pace, in particular in new and thus far poorly investigated non-model and environmentally-relevant species.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bacterias
/
Transporte de Electrón
/
Compuestos de Amonio
/
Anaerobiosis
/
Membranas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
2016
Tipo del documento:
Article