Development of fast and simple chromogenic methods for glucan phosphatases in-gel activity assays.
Anal Biochem
; 517: 36-39, 2017 Jan 15.
Article
en En
| MEDLINE
| ID: mdl-27836260
ABSTRACT
Glucan phosphatases are essential for normal starch degradation in plants and glycogen metabolism in mammals. Here we develop two chromogenic methods for the detection of glucan phosphatase activity in situ after non denaturing poliacrylamide gel electrophoresis; one method uses pNPP and the second one applies BCIP/NBT. The assays are sensitive, fast, simple, reliable and cost-effective preventing the use of radioactive or fluorogenic compounds. Taking advantage of an efficient separation method combined with the reported assays it is possible to obtain information about oligomeric state of the active enzymes as well as to simultaneously detect glucan substrate binding and phosphatase activity.
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Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
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Arabidopsis
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Proteínas de Arabidopsis
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Fosforilasas
Idioma:
En
Revista:
Anal Biochem
Año:
2017
Tipo del documento:
Article
País de afiliación:
Argentina