Identification of a rhodanese-like protein involved in thiouridine biosynthesis in Thermus thermophilus tRNA.
FEBS Lett
; 590(24): 4628-4637, 2016 Dec.
Article
en En
| MEDLINE
| ID: mdl-27878988
ABSTRACT
Incorporation of a sulfur atom into 2-thioribothymidine (s2 T or 5-methyl-2-thiouridine) at position 54 in thermophile tRNA is accomplished by an elaborate system composed of many proteins which confers thermostability to the translation system. We identified ttuD (tRNA-two-thiouridine D) as a gene for the synthesis of s2 T54 in Thermus thermophilus. The rhodanese-like protein TtuD enhances the activity of cysteine desulfurases and receives the persulfide generated by cysteine desulfurases in vitro. TtuD also enhances the formation of thiocarboxylated TtuB, the sulfur donor for the tRNA sulfurtransferase TtuA. Since cysteine desulfurases are the first enzymes in the synthesis of s2 T and other sulfur-containing compounds, TtuD has a role to direct sulfur flow to s2 T synthesis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sulfurtransferasas
/
Tiouridina
/
Proteínas Bacterianas
/
ARN de Transferencia
/
Thermus thermophilus
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
FEBS Lett
Año:
2016
Tipo del documento:
Article
País de afiliación:
Japón