Characterization of a triple-recombinant, reassortant rotavirus strain from the Dominican Republic.
J Gen Virol
; 98(2): 134-142, 2017 02.
Article
en En
| MEDLINE
| ID: mdl-27983480
ABSTRACT
We report the genome of a novel human triple-recombinant G4P[6-8_R] mono-reassortant strain identified in a stool sample from the Dominican Republic during routine facility-based rotavirus strain surveillance. The strain was designated as RVA/Human-wt/DOM/2013840364/2013/G4P[6-8_R], with a genomic constellation of G4-P[6-8_R]-I1-R1-C1-M1-(A1-A8_R)-N1-(T1-T7_R)-E1-H1. Recombinant gene segments NSP1 and NSP3 were generated as a result of recombination between genogroup 1 rotavirus A1 human strain and a genotype A8 porcine strain and between genogroup 1 rotavirus T1 human strain and a genotype T7 bovine strain, respectively. Analyses of the RNA secondary structures of gene segment VP4, NSP1 and NSP3 showed that all the recombinant regions appear to start in a loop (single-stranded) region and terminate in a stem (double-stranded) structure. Also, the VP7 gene occupied lineage VII within the G4 genotypes consisting of mostly porcine or porcine-like G4 strains, suggesting the occurrence of reassortment. The remaining gene segments clustered phylogenetically with genogroup 1 strains. This exchange of whole or partial genetic materials between rotaviruses by recombination and reassortment contributes directly to their diversification, adaptation and evolution.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Recombinación Genética
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Infecciones por Rotavirus
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Virus Reordenados
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Genoma Viral
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Rotavirus
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Gastroenteritis
Tipo de estudio:
Screening_studies
Límite:
Animals
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Humans
País/Región como asunto:
Caribe ingles
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Dominica
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Republica dominicana
Idioma:
En
Revista:
J Gen Virol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos