Pex35 is a regulator of peroxisome abundance.
J Cell Sci
; 130(4): 791-804, 2017 02 15.
Article
en En
| MEDLINE
| ID: mdl-28049721
ABSTRACT
Peroxisomes are cellular organelles with vital functions in lipid, amino acid and redox metabolism. The cellular formation and dynamics of peroxisomes are governed by PEX genes; however, the regulation of peroxisome abundance is still poorly understood. Here, we use a high-content microscopy screen in Saccharomyces cerevisiae to identify new regulators of peroxisome size and abundance. Our screen led to the identification of a previously uncharacterized gene, which we term PEX35, which affects peroxisome abundance. PEX35 encodes a peroxisomal membrane protein, a remote homolog to several curvature-generating human proteins. We systematically characterized the genetic and physical interactome as well as the metabolome of mutants in PEX35, and we found that Pex35 functionally interacts with the vesicle-budding-inducer Arf1. Our results highlight the functional interaction between peroxisomes and the secretory pathway.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Peroxisomas
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Proteínas de Saccharomyces cerevisiae
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Proteínas de la Membrana
Idioma:
En
Revista:
J Cell Sci
Año:
2017
Tipo del documento:
Article
País de afiliación:
Israel