Three-dimensional imaging flow cytometry through light-sheet fluorescence microscopy.
Cytometry A
; 91(2): 144-151, 2017 02.
Article
en En
| MEDLINE
| ID: mdl-28075531
ABSTRACT
Flow cytometry is the tool of choice for high-speed acquisition and analysis of large cell populations, with the tradeoff of lacking intracellular spatial information. Although in the last decades flow cytometry systems that can actually acquire two-dimensional spatial information were developed, some of the limitations remained though, namely constrains related to sample size and lack of depth or dynamic information. The combination of fluidics and light-sheet illumination has the potential to address these limitations. By having cells travelling with the flowing sheath one can, in a controlled fashion, force them at constant speed through the light-sheet enabling the synchronized acquisition of several optical sections, that is, three-dimensional imaging. This approach has already been used for imaging cellular spheroids, plankton, and zebra-fish embryos. In this review, we discuss the known solutions and standing challenges of performing three-dimensional high-throughput imaging of multicellular biological models using fluidics, while retaining cell and organelle-level resolution. © 2017 International Society for Advancement of Cytometry.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Imagenología Tridimensional
/
Citometría de Flujo
/
Microscopía Fluorescente
Límite:
Animals
Idioma:
En
Revista:
Cytometry A
Año:
2017
Tipo del documento:
Article
País de afiliación:
Portugal