[Determination of some androgens and anabolic steroids in human urine by HPLC].
Yao Xue Xue Bao
; 24(3): 207-11, 1989.
Article
en Zh
| MEDLINE
| ID: mdl-2816377
ABSTRACT
A simple method for determining androgens and anabolic steroids in human urine by HPLC has been developed. The compounds studied are nandrolone, methandienone, testosterone, methyltestosterone, testosterone propionate and nandrolone phenylpropionate. The stationary phase used is C8 bonded silica. Isocratic elution was done with CH3OH-CH3CN-H2O (456) and programmed flow. Detection limit can be less than 1 ng at the wavelength of 254 nm. The standard curves for each steroid have been set using internal standard (progesterone) and peak height ratio. Linear relationship exists between the ratio and concentration for each steroid. High and stable recovery has been achieved using Sep-Pak C18 cartridges for urine sample clean-up. The operation of the method is simple. The enzymatic hydrolyzation of conjugated steroids in human urine has also been investigated.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Anabolizantes
/
Andrógenos
Límite:
Humans
Idioma:
Zh
Revista:
Yao Xue Xue Bao
Año:
1989
Tipo del documento:
Article