Bone marrow-derived mesenchymal stem cells mitigate caspase-3 and 8-hydroxy proline induced via ß-adrenergic agonist in pulmonary injured rats.

ABSTRACT

Estimating the ability of bone marrow-derived mesenchymal stem cells (BM-MSCs) to alleviate pulmonary injury induced via isoproterenol (ISP). ISP was injected in a dose of (100 mg/kg, subcutaneously twice at an interval of 24 h). One month post BM-MSCs transplantation by intravenous injection, pulmonary oxidative stress was assessed, and Western blot analyses and histopathological investigations were conducted. Compared with the normal control group, BM-MSCs transplantation significantly decreased the expression of pulmonary anti-oxidative stress marker. Western blot analysis revealed that ISP significantly reduced the protein expression of the anti-oxidative stress marker nuclear related factor-2 (Nrf2). However, the apoptotic marker (caspase-3) and collagen content marker (8-hydroxyproline) were markedly elevated. These biochemical markers were confirmed by histopathological investigations. Finally, it was demonstrated that BM-MSCs transplantation showed a superior effect in improving pulmonary function through alleviating oxidative stress, apoptosis, and collagen content.