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Combined Haplotypes of CaSR Gene Sequence Variants and Their Associations with Growth Traits in Cattle.
Yue, Binglin; Han, Fuhai; Wu, Jiyao; Wang, Yanhuan; Zhang, Chunlei; Fang, Xingtang; Qi, Xinglei; Bai, Yueyu; Chen, Hong.
Afiliación
  • Yue B; a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
  • Han F; a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
  • Wu J; a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
  • Wang Y; a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
  • Zhang C; a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
  • Fang X; a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
  • Qi X; b Bureau of Animal Husbandry of Biyang County , Biyang , Henan , China.
  • Bai Y; c Animal Health Supervision in Henan Province , Zhengzhou , Henan , China.
  • Chen H; a Institute of Cellular and Molecular Biology, School of Life Science , Jiangsu Normal University , Xuzhou , Jiangsu , China.
Anim Biotechnol ; 28(4): 260-267, 2017 Oct 02.
Article en En | MEDLINE | ID: mdl-28267410
ABSTRACT
The calcium-sensing receptor (CaSR) is a Class C G-protein coupled receptor that regulates food intake and assimilation. However, studies on the relationship between CaSR gene and growth traits in cattle are deficient. The aim of this study was to examine the association of the CaSR polymorphism with growth traits in cattle breeds. Four novel single nucleotide polymorphisms (SNPs) and one previously reported SNP (NC_007299.5 g.67630865T>C, 67638409G>C, 67660395G>C, 67661546C>G, and 67661892A>C) were identified in the bovine CaSR gene using DNA sequencing and PCR-SSCP methods in 520 individuals from three representative breeds. The three SNP P4_2, P7_1, and P7_4 in LX, QC, and JX cattle populations belonged to intermediate genetic diversity (0.25 < PIC < 0.5). In addition, we evaluated the haplotype frequency and linkage disequilibrium coefficient of five sequence variants in the three cattle breeds. LD and haplotype structure of CaSR were different between breeds. LD analysis showed that the P4_2 and P7_4 loci were in complete LD in JX cattle population (r2 = 0.99 and D' = 1). Only 11 haplotypes were listed except for those with a frequency of <0.03. Hap1 (-TGGGC-) had the highest haplotype frequencies in LX (27.30%), Hap6(-TGGCC-) had the highest haplotype frequencies in QC (21.70%) and JX (32.30%). Association analysis indicated that P2, P4_2, and P7_4 loci were all significantly associated with growth traits and combined genotype TTGCGC was highly significantly associated with Chest circumference and body weight than the other genotype in JX cattle population. The results of this study suggest that the CaSR gene possibly is a strong candidate gene that affects growth traits in the Chinese cattle breeding program.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Haplotipos / Bovinos / Receptores Sensibles al Calcio Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: Anim Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Haplotipos / Bovinos / Receptores Sensibles al Calcio Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: Anim Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: China