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5-Aminolevulinic acid production from inexpensive glucose by engineering the C4 pathway in Escherichia coli.
Ding, Wenwen; Weng, Huanjiao; Du, Guocheng; Chen, Jian; Kang, Zhen.
Afiliación
  • Ding W; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
  • Weng H; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China.
  • Du G; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
  • Chen J; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China.
  • Kang Z; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
J Ind Microbiol Biotechnol ; 44(8): 1127-1135, 2017 08.
Article en En | MEDLINE | ID: mdl-28382525
ABSTRACT
5-Aminolevulinic acid (ALA), the first committed intermediate for natural biosynthesis of tetrapyrrole compounds, has recently drawn intensive attention due to its broad potential applications. In this study, we describe the construction of recombinant Escherichia coli strains for ALA production from glucose via the C4 pathway. The hemA gene from Rhodobacter capsulatus was optimally overexpressed using a ribosome binding site engineering strategy, which enhanced ALA production substantially from 20 to 689 mg/L. Following optimization of biosynthesis pathways towards coenzyme A and precursor (glycine and succinyl-CoA), and downregulation of hemB expression, the production of ALA was further increased to 2.81 g/L in batch-fermentation.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Microbiología Industrial / Microorganismos Modificados Genéticamente / Glucosa / Ácido Aminolevulínico Idioma: En Revista: J Ind Microbiol Biotechnol Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2017 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Microbiología Industrial / Microorganismos Modificados Genéticamente / Glucosa / Ácido Aminolevulínico Idioma: En Revista: J Ind Microbiol Biotechnol Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2017 Tipo del documento: Article