Molecular Imaging of Human Embryonic Stem Cells Stably Expressing Human PET Reporter Genes After Zinc Finger Nuclease-Mediated Genome Editing.
J Nucl Med
; 58(10): 1659-1665, 2017 10.
Article
en En
| MEDLINE
| ID: mdl-28596158
ABSTRACT
Molecular imaging is indispensable for determining the fate and persistence of engrafted stem cells. Standard strategies for transgene induction involve the use of viral vectors prone to silencing and insertional mutagenesis or the use of nonhuman genes. Methods:
We used zinc finger nucleases to induce stable expression of human imaging reporter genes into the safe-harbor locus adeno-associated virus integration site 1 in human embryonic stem cells. Plasmids were generated carrying reporter genes for fluorescence, bioluminescence imaging, and human PET reporter genes.Results:
In vitro assays confirmed their functionality, and embryonic stem cells retained differentiation capacity. Teratoma formation assays were performed, and tumors were imaged over time with PET and bioluminescence imaging.Conclusion:
This study demonstrates the application of genome editing for targeted integration of human imaging reporter genes in human embryonic stem cells for long-term molecular imaging.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Genoma Humano
/
Dedos de Zinc
/
Genes Reporteros
/
Tomografía de Emisión de Positrones
/
Endorribonucleasas
/
Células Madre Embrionarias
/
Edición Génica
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
J Nucl Med
Año:
2017
Tipo del documento:
Article
País de afiliación:
Bélgica