Adaptive optics improves multiphoton super-resolution imaging.
Nat Methods
; 14(9): 869-872, 2017 Sep.
Article
en En
| MEDLINE
| ID: mdl-28628128
ABSTRACT
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths â¼250 µm from the coverslip surface.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Aumento de la Imagen
/
Microscopía de Fluorescencia por Excitación Multifotónica
/
Lentes
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos