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Adaptive optics improves multiphoton super-resolution imaging.
Zheng, Wei; Wu, Yicong; Winter, Peter; Fischer, Robert; Nogare, Damian Dalle; Hong, Amy; McCormick, Chad; Christensen, Ryan; Dempsey, William P; Arnold, Don B; Zimmerberg, Joshua; Chitnis, Ajay; Sellers, James; Waterman, Clare; Shroff, Hari.
Afiliación
  • Zheng W; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA.
  • Wu Y; Research Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
  • Winter P; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA.
  • Fischer R; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA.
  • Nogare DD; National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
  • Hong A; Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
  • McCormick C; National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
  • Christensen R; Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
  • Dempsey WP; Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA.
  • Arnold DB; Department of Biology, Section of Molecular and Computational Biology, University of Southern California, Los Angeles, California, USA.
  • Zimmerberg J; Department of Biology, Section of Molecular and Computational Biology, University of Southern California, Los Angeles, California, USA.
  • Chitnis A; Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
  • Sellers J; Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
  • Waterman C; National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
  • Shroff H; National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
Nat Methods ; 14(9): 869-872, 2017 Sep.
Article en En | MEDLINE | ID: mdl-28628128
ABSTRACT
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ∼250 µm from the coverslip surface.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Aumento de la Imagen / Microscopía de Fluorescencia por Excitación Multifotónica / Lentes Tipo de estudio: Diagnostic_studies Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Aumento de la Imagen / Microscopía de Fluorescencia por Excitación Multifotónica / Lentes Tipo de estudio: Diagnostic_studies Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos