Improved sensitivity of firefly luminescent intermediate-based protein interaction assay using Ser 440 mutant with lower adenylation activity.

Ohmuro-Matsuyama, Yuki; Yamashita, Takahiro; Lin, Huan; Yamaji, Hideki; Ueda, Hiroshi

Ohmuro-Matsuyama, Yuki; Yamashita, Takahiro; Lin, Huan; Yamaji, Hideki; Ueda, Hiroshi.

Afiliación

Ohmuro-Matsuyama Y; Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.
Yamashita T; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada-ku, Kobe, Japan.
Lin H; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Yamaji H; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Ueda H; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada-ku, Kobe, Japan.

Luminescence ; 33(1): 125-130, 2018 Feb.

Article en En | MEDLINE | ID: mdl-28752574

Asunto(s)

Luciferasas de Luciérnaga/química; Luminiscencia; Serina/genética; Animales; Luciérnagas; Luciferasas de Luciérnaga/genética; Luciferasas de Luciérnaga/metabolismo; Mutación; Unión Proteica; Serina/metabolismo

Palabras clave

conformational change; firefly luciferase; protein-protein interaction

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Serina / Luciferasas de Luciérnaga / Luminiscencia Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Luminescence Asunto de la revista: BIOFISICA / BIOQUIMICA Año: 2018 Tipo del documento: Article País de afiliación: Japón

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