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Exposure to BMAA mirrors molecular processes linked to neurodegenerative disease.
Beri, Joshua; Nash, Tara; Martin, Rubia M; Bereman, Michael S.
Afiliación
  • Beri J; Department of Chemistry, North Carolina State University, Raleigh, NC, USA.
  • Nash T; Center for Human Health and the Environment, North Carolina State University, Raleigh, NC, USA.
  • Martin RM; Department of Biological Sciences, North Carolina State University, Raleigh, NC, USA.
  • Bereman MS; Department of Chemistry, North Carolina State University, Raleigh, NC, USA.
Proteomics ; 17(17-18)2017 Sep.
Article en En | MEDLINE | ID: mdl-28837265
The goal of this study is to investigate the molecular pathways perturbed by in vitro exposure of beta-methylamino-L-alanine (BMAA) to NSC-34 cells via contemporary proteomics. Our analysis of differentially regulated proteins reveals significant enrichment (p < 0.01) of pathways related to ER stress, protein ubiquitination, the unfolded protein response, and mitochondrial dysfunction. Upstream regulator analysis indicates that exposure to BMAA induces activation of transcription factors (X-box binding protein 1; nuclear factor 2 erythroid like 2; promyelocytic leukemia) involved in regulation of the UPR, oxidative stress, and cellular senescence. Furthermore, the authors examine the hypothesis that BMAA causes protein damage via misincorporation in place of L-Serine. The authors are unable to detect misincorporation of BMAA into protein via analysis of cellular protein, secreted protein, targeted detection of BMAA after protein hydrolysis, or through the use of in vitro protein translation kits.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Regulación de la Expresión Génica / Agonistas de Aminoácidos Excitadores / Aminoácidos Diaminos / Esclerosis Amiotrófica Lateral / Neuronas Motoras / Neuroblastoma Límite: Animals Idioma: En Revista: Proteomics Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Regulación de la Expresión Génica / Agonistas de Aminoácidos Excitadores / Aminoácidos Diaminos / Esclerosis Amiotrófica Lateral / Neuronas Motoras / Neuroblastoma Límite: Animals Idioma: En Revista: Proteomics Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos