Determination of cholesterol and four phytosterols in foods without derivatization by gas chromatography-tandem mass spectrometry.
J Food Drug Anal
; 23(4): 636-644, 2015 Dec.
Article
en En
| MEDLINE
| ID: mdl-28911479
ABSTRACT
In this study, a method for determination of cholesterol and four phytosterols by gas chromatography coupled with electron impact ionization mode-tandem mass spectrometry without derivatization in general food was developed. The sample was saponified with 7.5% KOH in methanol. After heating on hot plate and reflux for 60 minutes, the saponified portion was extracted with n-hexane/petroleum ether (5050, v/v). The extracts were evaporated with rotary evaporator and then redissolved with tetrahydrofuran. The tetrahydrofuran layer was transferred into an injection vial and analyzed by gas chromatography on a 30 m VF-5 column. Limit of quantification was 2 mg/kg. Recoveries of cholesterol and four phytosterols from general food were between 91% and 100%.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
J Food Drug Anal
Año:
2015
Tipo del documento:
Article
País de afiliación:
Taiwán