Whole-Genome Shotgun Sequencing of Two ß-Proteobacterial Species in Search of the Bulgecin Biosynthetic Cluster.
ACS Chem Biol
; 12(10): 2552-2557, 2017 10 20.
Article
en En
| MEDLINE
| ID: mdl-28937735
ABSTRACT
We have produced draft whole-genome sequences for two bacterial strains reported to produce the bulgecins as well as NRPS-derived monobactam ß-lactam antibiotics. We propose classification of ATCC 31363 as Paraburkholderia acidophila. We further reaffirm that ATCC 31433 (Burkholderia ubonensis subsp. mesacidophila) is a taxonomically distinct producer of bulgecins with notable gene regions shared with Paraburkholderia acidophila. We use RAST multiple-gene comparison and MASH distancing with published genomes to order the draft contigs and identify unique gene regions for characterization. Forty-eight natural-product gene clusters are presented from PATRIC (RASTtk) and antiSMASH annotations. We present evidence that the 10 genes that follow the sulfazecin and isosulfazecin pathways in both species are likely involved in bulgecin A biosynthesis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Glicopéptidos
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Regulación Bacteriana de la Expresión Génica
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Genoma Bacteriano
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Burkholderiaceae
Idioma:
En
Revista:
ACS Chem Biol
Año:
2017
Tipo del documento:
Article