[Correlation of oxidative stress with sperm DNA integrity and semen parameters in infertile men with varicocele].
Zhonghua Nan Ke Xue
; 22(6): 530-533, 2016 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-28963843
ABSTRACT
OBJECTIVE:
To investigate the relationship of oxidative stress with DNA integrity and semen parameters in infertile men with varicocele (VC).METHODS:
This prospective study included 98 infertile males with VC. According to the levels of reactive oxygen species (ROS) in the semen, we divided the patients into a high ROS group (n=44) and a low ROS group (n=54), determined the sperm DNA fragmentation index (DFI), motility and morphology, and analyzed their correlation with ROS in the two groups of patients.RESULTS:
Compared with the patients of the low ROS group, those of the high ROS group showed a significantly higher DFI (27.38±8.10 vs 34.49±6.05, P=0.039) and a higher concentration of seminal leukocytes (ï¼»0.65±0.15ï¼½×106/ml vs ï¼»0.86±0.41ï¼½×106/ml, P=0.022), but lower sperm motility (ï¼»36.16±22.83ï¼½% vs ï¼»18.22±25.21ï¼½%, P=0.017), percentage of progressively motile sperm (ï¼»23.34±11.53ï¼½% vs ï¼»16.34±9.22ï¼½%, P=0.041), sperm curvilinear velocity (ï¼»27.03±6.21ï¼½ vs ï¼»20.62±4.38ï¼½ µm/s, P=0.013), and sperm linearity (ï¼»29.75±8.24ï¼½% vs ï¼»18.30±7.93ï¼½%, P=0.024). Spearman correlation analysis indicated that the ROS level was correlated positively with the concentration of seminal leukocytes (r=0.41, P<0.01) and DFI (r=0.21, P=0.006), but negatively with sperm curvilinear velocity (r=ï¼0.24, P=0.017), linearity (r=ï¼0.24, P=0.021), motility (r=ï¼0.31, P=0.002), and the percentage of progressively motile sperm (r=ï¼0.41, P=0.012). Additionally, the sperm DFI manifested a significant negative correlation with sperm motility (r=ï¼0.29, P<0.01) and the percentage of progressively motile sperm (r=ï¼0.34, P<0.01).CONCLUSIONS:
The level of seminal ROS is positively correlated with the sperm DFI in infertile men with varicocele, and both the ROS level and DNA integrity are associated with semen parameters.Palabras clave
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
Varicocele
/
Estrés Oxidativo
/
Fragmentación del ADN
/
Infertilidad Masculina
Tipo de estudio:
Observational_studies
Límite:
Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua Nan Ke Xue
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2016
Tipo del documento:
Article
País de afiliación:
China