Metabolome and transcriptome profiling reveal new insights into somatic embryo germination in Norway spruce (Picea abies).
Tree Physiol
; 37(12): 1752-1766, 2017 12 01.
Article
en En
| MEDLINE
| ID: mdl-28985382
ABSTRACT
Transcriptome, metabolome and histological profiling were performed on normal and aberrant somatic embryo germinants of Norway spruce (Picea abies L. Karst) providing a simplistic systems biology description of conifer germination. Aberrant germinants (AGs) formed periderm-like tissue at the apical pole and lacked shoot growth above the cotyledons. Transcriptome profiling (RNA-Sequencing) revealed a total of 370 differentially expressed genes at ≥1 or ≤-1 log2-fold change, where 92% were down-regulated in AGs compared with normal germinants (NGs). Genes associated with shoot apical meristem formation were down-regulated in AGs, or not differentially expressed between AGs and NGs. Genes involved in hormone signaling and transport were also down-regulated. Metabolite profiling by gas chromatography-mass spectrometry (MS) and liquid chromatography-MS revealed biochemical difference between AGs and NGs, notably increased levels of sugars including glucose in AGs. Genes involved in glucose signaling were down-regulated and genes involved in starch biosynthesis were up-regulated, suggesting involvement of sugar signaling during late embryo development and germination. The overall results provide new data enabling further studies to confirm potential markers for a normal germination process in conifers.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Semillas
/
Picea
/
Metaboloma
/
Transcriptoma
Idioma:
En
Revista:
Tree Physiol
Asunto de la revista:
BOTANICA
/
FISIOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Suecia