Your browser doesn't support javascript.
loading
Effects of the pH and Concentration on the Stability of Standard Solutions of Proteinogenic Amino Acid Mixtures.
Kato, Megumi; Yamazaki, Taichi; Kato, Hisashi; Yamanaka, Noriko; Takatsu, Akiko; Ihara, Toshihide.
Afiliación
  • Kato M; National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST).
  • Yamazaki T; National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST).
  • Kato H; National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST).
  • Yamanaka N; National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST).
  • Takatsu A; National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST).
  • Ihara T; National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST).
Anal Sci ; 33(11): 1241-1245, 2017.
Article en En | MEDLINE | ID: mdl-29129862
ABSTRACT
To prepare metrologically traceable amino acid mixed standard solutions, it is necessary to determine the stability of each amino acid present in the mixed solutions. In the present study, we prepared amino acid mixed solutions using certified reference standards of 17 proteinogenic amino acids, and examined the stability of each of these amino acids in 0.1 N HCl. We found that the concentration of glutamic acid decreased significantly during storage. LC/MS analysis indicated that the instability of glutamic acid was due to the partial degradation of glutamic acid to pyroglutamic acid in 0.1 N HCl. Using accelerated degradation tests, we investigated several solvent compositions to improve the stability of glutamic acid in amino acid mixed solution, and determined that the change of the pH by diluting the mixed solution improved the stability of glutamic acid.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas / Ácido Glutámico / Almacenaje de Medicamentos Idioma: En Revista: Anal Sci Año: 2017 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas / Ácido Glutámico / Almacenaje de Medicamentos Idioma: En Revista: Anal Sci Año: 2017 Tipo del documento: Article