Dynamics of Indel Profiles Induced by Various CRISPR/Cas9 Delivery Methods.

Kosicki, Michael; Rajan, Sandeep S; Lorenzetti, Flaminia C; Wandall, Hans H; Narimatsu, Yoshiki; Metzakopian, Emmanouil; Bennett, Eric P

Kosicki, Michael; Rajan, Sandeep S; Lorenzetti, Flaminia C; Wandall, Hans H; Narimatsu, Yoshiki; Metzakopian, Emmanouil; Bennett, Eric P.

Afiliación

Kosicki M; Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom.
Rajan SS; Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom.
Lorenzetti FC; Departments of Cellular and Molecular Medicine and Odontology, Copenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark.
Wandall HH; Departments of Cellular and Molecular Medicine and Odontology, Copenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark.
Narimatsu Y; Departments of Cellular and Molecular Medicine and Odontology, Copenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark; GlycoDisplay Aps, Copenhagen, Denmark.
Metzakopian E; Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom. Electronic address: em11@sanger.ac.uk.
Bennett EP; Departments of Cellular and Molecular Medicine and Odontology, Copenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark. Electronic address: epb@sund.ku.dk.

Prog Mol Biol Transl Sci ; 152: 49-67, 2017.

Article en En | MEDLINE | ID: mdl-29150004

Asunto(s)

Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas; Mutación INDEL; Reparación del ADN; Humanos

Palabras clave

CRISPR/Cas9; PiggyBac; endonuclease mismatch cleavage (EMC) assay; indel detection by amplicon analysis (IDAA); indel profiles and dynamics; lentiviral; next generation sequencing (NGS); plasmid delivery; ribonuclear protein (RNP); stabilized synthetic SygRNA(TM) crRNA and tracrRNA; tracking of indels by decomposition (TIDE)

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Mutación INDEL / Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas Límite: Humans Idioma: En Revista: Prog Mol Biol Transl Sci Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Reino Unido

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