Dihydroartemisinin up-regulates VE-cadherin expression in human renal glomerular endothelial cells.

Li, Liqun; Chen, Xiaocui; Dong, Fengyun; Liu, Qiang; Zhang, Caiqing; Xu, Dongmei; Allen, Thaddeus D; Liu, Ju

Li, Liqun; Chen, Xiaocui; Dong, Fengyun; Liu, Qiang; Zhang, Caiqing; Xu, Dongmei; Allen, Thaddeus D; Liu, Ju.

Afiliación

Li L; Laboratory of Microvascular Medicine, Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, China.
Chen X; Laboratory of Microvascular Medicine, Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, China.
Dong F; Laboratory of Microvascular Medicine, Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, China.
Liu Q; Laboratory of Microvascular Medicine, Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, China.
Zhang C; Department of Respiratory and Critical Care Medicine, Shandong Provincial Qianfoshan Hospital, Shandong, University, Jinan, Shandong, China.
Xu D; Department of Nephrology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, China.
Allen TD; Tradewind BioScience, Daly City, CA, USA.
Liu J; Laboratory of Microvascular Medicine, Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, China.

J Cell Mol Med ; 22(3): 2028-2032, 2018 03.

Article en En | MEDLINE | ID: mdl-29193726

ABSTRACT

ABSTRACT

The antimalarial agent dihydroartemisinin (DHA) has been shown to be anti-inflammatory. In this study, we found that DHA increased the expression of the junctional protein vascular endothelial (VE)-cadherin in human renal glomerular endothelial cells. In addition, DHA inhibited TGF-ß RI-Smad2/3 signalling and its downstream effectors SNAIL and SLUG, which repress VE-cadherin gene transcription. Correspondingly, DHA decreased the binding of SNAIL and SLUG to the VE-cadherin promoter. Together, our results suggest an effect of DHA in regulating glomerular permeability by elevation of VE-cadherin expression.

Asunto(s)

Antiinflamatorios no Esteroideos/farmacología; Antígenos CD/genética; Artemisininas/farmacología; Cadherinas/genética; Células Endoteliales/efectos de los fármacos; Proteína Smad2/genética; Factor de Crecimiento Transformador beta1/genética; Antígenos CD/metabolismo; Antimaláricos/farmacología; Cadherinas/agonistas; Cadherinas/metabolismo; Línea Celular; Reposicionamiento de Medicamentos; Células Endoteliales/citología; Células Endoteliales/metabolismo; Regulación de la Expresión Génica; Humanos; Glomérulos Renales/citología; Glomérulos Renales/efectos de los fármacos; Glomérulos Renales/metabolismo; Regiones Promotoras Genéticas; Unión Proteica; Transducción de Señal; Proteína Smad2/antagonistas & inhibidores; Proteína Smad2/metabolismo; Proteína smad3/genética; Proteína smad3/metabolismo; Factores de Transcripción de la Familia Snail/genética; Factores de Transcripción de la Familia Snail/metabolismo; Factor de Crecimiento Transformador beta1/antagonistas & inhibidores; Factor de Crecimiento Transformador beta1/metabolismo

Palabras clave

TGF-ß signalling; VE-cadherin; dihydroartemisinin; glomerular endothelial cells

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Antígenos CD / Cadherinas / Antiinflamatorios no Esteroideos / Artemisininas / Células Endoteliales / Proteína Smad2 / Factor de Crecimiento Transformador beta1 Límite: Humans Idioma: En Revista: J Cell Mol Med Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article País de afiliación: China

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