miRNA-20a upregulates TAK1 and increases proliferation in osteosarcoma cells.
Future Oncol
; 14(5): 461-469, 2018 Feb.
Article
en En
| MEDLINE
| ID: mdl-29327611
ABSTRACT
AIM:
The aim of this study is to explore the function of miR-20a in osteosarcoma. MATERIALS &METHODS:
miR-20a expression was measured by real-time PCR. miR-20a mimics, inhibitor and scramble siRNA were transfected into osteosarcoma cells to observe effects on colony formation and tumor growth. Moreover, relationships of miR-20a with TAK1 were investigated by western blot and luciferase activity.RESULTS:
We found that miR-20a was downregulated in osteosarcoma, and overexpression of miR-20a reduced colony formation and tumor growth. Furthermore, the data revealed that the function of miR-20a was probably exerted via targeting the TAK1 expression. Overexpression of miR-20a sensitizes the osteosarcoma cells to chemotherapeutic drugs.CONCLUSION:
Our data identify the role of miR-20a in osteosarcoma growth, indicating its potential application in chemotherapy.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias Óseas
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Osteosarcoma
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Quinasas Quinasa Quinasa PAM
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MicroARNs
/
Interferencia de ARN
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Future Oncol
Año:
2018
Tipo del documento:
Article
País de afiliación:
China