Two Diterpene Synthases for Spiroalbatene and Cembreneâ
A from Allokutzneria albata.
Angew Chem Int Ed Engl
; 57(12): 3238-3241, 2018 03 12.
Article
en En
| MEDLINE
| ID: mdl-29377369
ABSTRACT
Two bacterial diterpene synthases from the actinomycete Allokutzneria albata were investigated, resulting in the identification of the structurally unprecedented compound spiroalbatene from the first and cembreneâ
A from the second enzyme. Both enzymes were thoroughly investigated in terms of their mechanisms by isotope labeling experiments, site-directed mutagenesis, and variation of the metal cofactors and pHâ
value. For spiroalbatene synthase, the pH- and Mn2+ -dependent formation of the side product thunbergol was observed, which is biosynthetically linked to spiroalbatene.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Actinomycetales
/
Transferasas Alquil y Aril
/
Diterpenos
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Año:
2018
Tipo del documento:
Article
País de afiliación:
Alemania