Carbonic anhydrase expression in the branchial ionocytes of rainbow trout.
J Exp Biol
; 221(Pt 5)2018 03 12.
Article
en En
| MEDLINE
| ID: mdl-29378818
ABSTRACT
Rainbow trout (Oncorhynchus mykiss) exposed to acid-base challenges activate branchial mechanisms for the excretion of acid-base equivalents. Current models of branchial acid-base excretion in freshwater rainbow trout propose two main ionocyte types the peanut lectin agglutinin-positive (PNA+) mitochondrion-rich cell or ionocyte is believed to secrete HCO3- in exchange for Cl-, whereas H+ secretion is thought to occur across PNA- ionocytes in exchange for Na+ Both HCO3- and H+ are supplied by intracellular hydration of CO2 catalysed by cytosolic carbonic anhydrase (CAc). Immunohistochemical approaches revealed that under control conditions, CAc was detectable in 92.3±1.0% (N=11) of PNA- ionocytes, and the abundance of PNA- ionocytes increased in response to systemic acidosis elicited by 72â
h exposure to water of low pH (nominally pH 4.5), hypercapnia (1% CO2, nominally 7.6 Torr) or hyperoxia (achieved by gassing water with pure O2), as did the abundance of PNA- ionocytes that exhibited immunofluorescence for CAc. However, just 4.3±0.6% (N=11) of PNA+ ionocytes expressed detectable CAc under control conditions. Marked increases in the abundance of CAc-positive PNA+ ionocytes were detected following exposure of trout to a base load via recovery from hypercapnia or base infusion (72â
h infusion with 140â
mmolâ
l-1 NaHCO3). The percentage of CAc-positive PNA+ ionocytes also was increased in trout treated with cortisol (10â
mgâ
kg-1 hydrocortisone 21-hemisuccinate daily for 7â
days). These results suggest that regulation of CA within PNA+ ionocytes and/or the abundance of CAc-positive PNA+ ionocytes plays a role in activating base secretion in response to systemic alkalosis.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Equilibrio Ácido-Base
/
Anhidrasas Carbónicas
/
Branquias
Límite:
Animals
Idioma:
En
Revista:
J Exp Biol
Año:
2018
Tipo del documento:
Article