Isolation of troponins from striated and smooth adductor muscles of Akazara scallop.
J Biochem
; 100(3): 821-4, 1986 Sep.
Article
en En
| MEDLINE
| ID: mdl-2946671
ABSTRACT
Troponins which confer Ca-sensitivity to skeletal actomyosin ATPase were successfully isolated from striated and smooth adductor muscles of "Akazara" scallop (Chlamys nipponensis akazara). SDS-gel electrophoresis showed that striated and smooth adductor troponins were composed of three components having molecular weights of about 52K (52,000), 40K, and 20K, and about 40K, 21K, and 20K, respectively. The Mg-ATPase activity of actomyosin reconstituted from rabbit actin and either Akazara striated adductor myosin or smooth adductor myosin, along with the respective tropomyosin and troponin, indicated that the Ca2+ concentration required for the activation of actomyosin ATPase appeared to be favorable to myosin-linked regulation.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Troponina
/
Moluscos
Límite:
Animals
Idioma:
En
Revista:
J Biochem
Año:
1986
Tipo del documento:
Article